Cancer is an ailment which gradually influences and brings down the life expectancy of human begins. There is an increasing exigency towards the aversion and remedy for this life threatening disease. Fortunately, most of the researches accentuate on naturally derived product, as they considered having less toxicity and less side effects when compared to the current treatment viz., chemotherapy. Actually, the most prevalent drug sources are natural drugs and can provide more structural diversity and novelty. So in the present study, the anti-proliferative potential of extracts of three Indian medicinal plants namely Ammania baccifera ,Azima tetracantha., Melothria maderaspatana were evaluated against human cervical cancer cell line (Hela)  by cytotoxic assay (MTT). The results of cellular viability in human cervical Cancer line (Hela) demonstrated a pronounced anti proliferative activity that may be attributed to the phyto constituent present in the extracts.